Welcome to my website and blog! I’m Larry Rodriguez, a PhD scientist, hiphop head, and award winning stand-up comedian. Check out my publications, learn more about my background and my projects, or check out my methods and resources section to see how I do things!
All tagged TEVC
I wanted to use this post to share the method I used to pre-emptively protect genes from degradation after RNA synthesis. Its not a perfect replacement for cloning, but if you’re short on time and need to protect you transcription products from RNAse degradation, this technique might be exactly what you need.
Working with mRNA requires you to work carefully and diligently. Here are some tips to help your electrophyisology studies, if you’re inject mRNA and having trouble with ion channel/receptor expression.
Our lab uses analog chart recorders to record data from two electrode voltage clamp electrophsiology experiments in Xenopus laevis oocyte because digitizers are expensive at over >$5000, but here is a low-cost <$300 solution!
